Corning® ITS Premix Universal Culture Supplement, 5 mL, 1/Pack

Corning® ITS Premix Universal Culture Supplement, 5 mL, contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media. The concentrated supplement stimulates cell proliferation of a variety of cells under serum-reduced conditions. 5 mL (5L equivalent), 1/pack

The ITS Premix (354350 and 354231) contains the following when used at the recommended dilution: Insulin (5ug/ mL), Transferrin (5ug/ mL), and Selenious Acid (5ng/ mL)

Formulation

Corning ITS Premix -Lyophilized from a water solution; reconstitute in 20 mL (cat. no. 354350) or 5 mL (cat. no. 354351) dH2O (stock solution).

Quality

• Tested for ability to promote proliferation of HeLa cells in serum-free medium
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Preparation and Storage

Corning ITS Premix: Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Avoid multiple freeze/thaws.

Tip

Corning ITS Premix - Recommended dilution - 1:1000 (dilute stock solution in serum-free medium)

The Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells.

• Vialed, highly purified growth factors with high biological activity
• Broad range of highly purified natural and recombinant lymphokines
• Enriched culture supplements to be reconstituted/added in the medium of your choice
• Propagate your cells under more defined serum-reduced or serum-free conditions

Corning offers a broad range of other media growth supplements. Some products are specific to cell type, such as SMC4 (for iPSCs) and T-Cell culture supplement (for T-cells), while other products promote proliferation in a wide variety of cell types. Reducing serum use is made easy with products such as ITS and Corning Nu-Serum™.